Proximate parameters - Proteins

Parameters:

Crude fat
Crude fibre
Crude protein
Moisture

Wet chemistry reference methods:

Reference methods used are as outlined in Premium Grains For Livestock Program. Component 1: Co-ordination. An overview of outcomes from PGLP 1 & 2. Final report. September 2008. Prepared by John Black.

Crude fat

A sample (2g) of ground (<1.0mm) seed was weighed into a soxhlet extraction thimble and extracted for eight hours with a constant boiling range (40-60°C) hydrocarbon solvent. The hydrocarbon solvent was removed and the amount of fat / oil was determined gravimetrically (AOAC, 1995, 4.5.01).

References:

AOAC (1995) Official Methods of Analysis of the Association of Official Analytical Chemists. Volume 1 and 2, 16th edition, Editor P. Cunniff, Published AOAC International, Arlington, Virginia, 4.1.16, 4.1.10, 4.2.04, 4.5.01, 4.6.01, 4.6.03, 4.6.03D, 32.5.18, 41.1.28, 41.1.29.

Tecnicon, Method Manual, Method 334-74.

Crude fibre

A ground (<1.0mm) seed sample (2g) was extracted with 10% w/v sulphuric acid and 10% w/v sodium hydroxide and the residue was collected in a glass sintered glass crucible. The crude fibre was determined gravimetrically according to the method in the Official Methods of Analysis of the AOAC (1995).

References:

Tecnicon, Method Manual, Method 334-74.

Crude protein

The finely milled (<1.0mm) seed sample (0.8g) was pressed into a pellet and underwent combustion in pure oxygen at 850°C. The amount of nitrogen was measured by thermal conductivity (Dumas Nitrogen, AOAC, 1995, 4.2.04). Crude protein was calculated by multiplying the nitrogen value by 6.25.

References:

Tecnicon, Method Manual, Method 334-74.

Moisture